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Proceedings Paper

Applications of fluorescence spectroscopy and dynamic light scattering in biotechnology of camptothecins: promising anticancer drugs
Author(s): Stefan Kruszewski; Thomas G. Burke
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Paper Abstract

Methods of optical measurements are widely used in biomedical research. Applications of these methods in determining of properties of camptothecins -- S-phase anticancer agents are described in this paper. Under physiological conditions CPT hydrolyses, and converts into inactive carboxylate form. Decrease of active form of CPT occurs especially fast in plasma of human blood. One of camptothecin analogues -- DB-67, exhibits, in contrary to CPT, relatively high stability in human blood. Fluorescence spectroscopy methods were used to determine physical and to predict biological properties of camptothecins, including DB-67. The dynamic light scattering (DLS) methods were applied to control the sizes of liposomes used as DB-67 delivery system.

Paper Details

Date Published: 1 April 2003
PDF: 9 pages
Proc. SPIE 5064, Lightmetry 2002: Metrology and Testing Techniques Using Light, (1 April 2003); doi: 10.1117/12.501386
Show Author Affiliations
Stefan Kruszewski, Univ. of Technology and Agriculture (Poland)
Ludwik Rydygier Medical Univ. (Poland)
Thomas G. Burke, Univ. of Kentucky (United States)

Published in SPIE Proceedings Vol. 5064:
Lightmetry 2002: Metrology and Testing Techniques Using Light
Maksymilian Pluta; Mariusz Szyjer, Editor(s)

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