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Proceedings Paper

Toward Fourier interferometry fluorescence excitation/emission imaging of malignant cells combined with photoacoustic microscopy
Author(s): Elli Kohen; Joseph G. Hirschberg; John P. Berry; Nuri Ozkutuk; Ceren Ornek; Marco Monti; Roger M. Leblanc; Dietrich O. Schachtschabel; Sumaira Haroon
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Paper Abstract

Dual excitation fluorescence imaging has been used as a first step towards multi-wavelength excitation/emission fluorescence spectral imaging. Target cells are transformed keratinocytes, and other osteosarcoma, human breast and color cancer cells. Mitochondrial membrane potential probes, e.g. TMRM (tetramethylrhodamine methyl ester), Mitotracker Green (Molecular Probes, Inc., Eugene OR,USA) and a recently synthesized mitochondrial oxygen probe, [PRE,P1"- pyrene butyl)-2-rhodamine ester] allow dual excitation in the UV plus in teh blue-green spectral regions. Also, using the natural endogenous probe NAD(P)H, preliminary results indicate mitochondrial responses to metabolic challenges (e.g. glucose addition), plus changes in mitochonrial distribution and morphology. In terms of application to biomedicine (for diagnostiscs, prognostsics and drug trials) three parameters have been selected in addition to the natural probe NAD(P)H, i.e. vital fluorescence probing of mitochondria, lysosomes and Golgi apparatus. It is hoped that such a multiparameter approach will allow malignant cell characterization and grading. A new area being introduced is the use of similar methodology for biotechnical applications such as the study of the hydrogen-producing alga Chlamydomonas Reinhardtii, and possible agricultural applications, such as Saccharomyces yeast for oenology. Complementation by Photoacoustic Microscopy is also contemplated, to study the internal conversion component which follows the excitation by photons.

Paper Details

Date Published: 14 October 2003
PDF: 11 pages
Proc. SPIE 5143, Novel Optical Instrumentation for Biomedical Applications, (14 October 2003); doi: 10.1117/12.500999
Show Author Affiliations
Elli Kohen, Univ. of Miami (United States)
Joseph G. Hirschberg, Univ. of Miami (United States)
John P. Berry, Univ. of Miami (United States)
Nuri Ozkutuk, Univ. of Manisa (Turkey)
Ceren Ornek, Univ. of Miami (United States)
Marco Monti, Univ. of Miami (United States)
Roger M. Leblanc, Univ. of Miami (United States)
Dietrich O. Schachtschabel, Philipps-Univ. Marburg (Germany)
Sumaira Haroon, Univ. of Miami (United States)

Published in SPIE Proceedings Vol. 5143:
Novel Optical Instrumentation for Biomedical Applications
Albert-Claude Boccara, Editor(s)

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