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Proceedings Paper

Intracellular photoinduced oxidative stress by zinc phthalocyanine photosensitization: a study of the early events in real time using confocal microscopy
Author(s): Eleni Alexandratou; Dido Yova; Panagiotis Handris; Dimitris Kletsas; Spyros Loukas
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Paper Abstract

Oxidative stress has been implicated in several biological and pathological aspects. Reactive oxygen species (ROS) have been proposed to act as signal transduction molecules activating reactions leading to cell rescue or to cell apoptosis/necrosis. In the present study, oxidative stress was induced by photosensitization of zinc phthalocyanine (ZnPc) in human fibroblasts using a photodynamic dose that did not lead to apoptosis or necrosis. The induction of oxidative stress was performed at the microscope stage in preassigned time. The cascade of phenomena evoked was studied in real time and at the single cell level using confocal laser scanning microscopy. Using specific vital fluorescent probes, alterations induced by oxidative stress in mitochondria membrane potential, in intracellular pH and in calcium concentration were recorded. Image processing and analysis techniques were used to quantify the observed changes. Subcellular localization of the photosensitizer was studied in order to determine the primary and immediate ROS target. It was found that ZnPc is mainly localized in the mitochondria region.

Paper Details

Date Published: 16 October 2003
PDF: 8 pages
Proc. SPIE 5142, Therapeutic Laser Applications and Laser-Tissue Interactions, (16 October 2003); doi: 10.1117/12.500597
Show Author Affiliations
Eleni Alexandratou, National Technical Univ. of Athens (Greece)
Dido Yova, National Technical Univ. of Athens (Greece)
Panagiotis Handris, National Ctr. for Scientific Research Demokritos (Greece)
Dimitris Kletsas, National Ctr. for Scientific Research Demokritos (Greece)
Spyros Loukas, National Ctr. for Scientific Research Demokritos (Greece)

Published in SPIE Proceedings Vol. 5142:
Therapeutic Laser Applications and Laser-Tissue Interactions
Rudolf W. Steiner, Editor(s)

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