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Proceedings Paper

Multiphoton imaging using a Ti:sapphire regenerative amplifier
Author(s): Patrick Theer; Mazahir T. Hasan; Winfried T. Denk
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Paper Abstract

It is shown that two-photon fluorescence images can be obtained almost throughout the entire grey-matter layers of the mouse neocortex by using optically amplified femtosecond pulses. The maximum imaging depth is not limited by the available excitation power but instead by the generation of out-of focus fluorescence.

Paper Details

Date Published: 9 October 2003
PDF: 6 pages
Proc. SPIE 5139, Confocal, Multiphoton, and Nonlinear Microscopic Imaging, (9 October 2003); doi: 10.1117/12.499783
Show Author Affiliations
Patrick Theer, Max-Planck-Institute for Medical Research (Germany)
Mazahir T. Hasan, Max-Planck-Institute for Medical Research (Germany)
Winfried T. Denk, Max-Planck-Institute for Medical Research (Germany)

Published in SPIE Proceedings Vol. 5139:
Confocal, Multiphoton, and Nonlinear Microscopic Imaging
Tony Wilson, Editor(s)

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