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Proceedings Paper

Methylation oligonucleotide microarray: a novel tool to analyze methylation patterns
Author(s): Peng Hou; Meiju Ji; Nongyao He; Zuhong Lu
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Paper Abstract

A new technique to analyze methylation patterns in several adjacent CpG sites was developed and reported here. We selected a 336bp segment of the 5’-untranslated region and the first exon of the p16Ink4a gene, which include the most densely packed CpG fragment of the islands containing 32 CpG dinucleotides, as the investigated target. The probes that include all types of methylation patterns were designed to fabricate a DNA microarray to determine the methylation patterns of seven adjacent CpG dinucleotides sites. High accuracy and reproducibility were observed in several parallel experiments. The results led us to the conclusion that the methylation oligonucleotide microarray can be applied as a novel and powerful tool to map methylation patterns and changes in multiple CpG island loci in a variety of tumors.

Paper Details

Date Published: 18 April 2003
PDF: 8 pages
Proc. SPIE 5119, Bioengineered and Bioinspired Systems, (18 April 2003); doi: 10.1117/12.497693
Show Author Affiliations
Peng Hou, Southeast Univ. (China)
Meiju Ji, Southeast Univ. (China)
Nongyao He, Southeast Univ. (China)
Zuhong Lu, Southeast Univ. (China)

Published in SPIE Proceedings Vol. 5119:
Bioengineered and Bioinspired Systems
Angel Rodriguez-Vazquez; Derek Abbott; Ricardo Carmona, Editor(s)

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