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Proceedings Paper

Confocal microscopic and spectroscopic investigation of autofluorescence of dictyosopharium pulchellum wood
Author(s): Narahari V. Joshi; Honorio Medina; Maria Vielma
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Paper Abstract

Dictyosphaerium puichellum Wood, a typical a fresh water alga, is known for photosynthesis and expected to have photoluminescence also. We have, therefore, carried out a confocal fluorescence microscopic and spectroscopic study of this species. 3-D image constructed from 50 optical sections clearly reveals that fluorescence is originated from only the outer layer and not from the central core. Spectral investigation shows that a strong and sharp peak is located at 684 nm. At this peak value, the power measurement was carried out and the number of photons emitted by alga in the solid angle 48.59 f was calculated and found to be 3.4*1010/ sec. The other significant feature of the responsible fluorophore is that the present photoluminescence has a very low quenching rate and hence the emitted radiation is observed for a very long time (5 12 sec.). This directly suggests that this fluorophore could have direct applications in dye related industries.

Paper Details

Date Published: 2 November 2001
PDF: 4 pages
Proc. SPIE 4431, Photon Migration, Optical Coherence Tomography, and Microscopy, (2 November 2001); doi: 10.1117/12.496590
Show Author Affiliations
Narahari V. Joshi, Univ. de Los Andes (Spain)
Honorio Medina, Univ. de Los Andes (Venezuela)
Maria Vielma, Univ. of Los Andes (Venezuela)

Published in SPIE Proceedings Vol. 4431:
Photon Migration, Optical Coherence Tomography, and Microscopy
Stefan Andersson-Engels; Michael F. Kaschke, Editor(s)

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