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Proceedings Paper

Biological photonic crystals revealed by multimodality nonlinear microscopy
Author(s): Chi-Kuang Sun; Shi-Wei Chu; I-Hsiu Chen; Bai-Ling Lin; Ping Chin Cheng
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Paper Abstract

A novel multi-modality nonlinear microscopy reveals highly optically-active biophotonic crystal structures in living cells. Numerous biological structures, including stacked membranes and arranged protein structures are highly organized in optical scale and are found to exhibit strong optical activities through second-harmonic-generation (SHG) interactions, behaving similar to man-made photonic crystals. The microscopic technology developed is based on a combination of imaging modalities including not only SHG, but also third-harmonic-generation and multi-photonfluorescence. With no energy deposition during harmonic generation processes, the demonstrated highly-penetrative yet non-invasive microscopy is useful for investigating the dynamics of structure-function relationship at the molecular and subcellular levels and is ideal for studying living cells that require minimal or no preparation.

Paper Details

Date Published: 17 June 2002
PDF: 9 pages
Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); doi: 10.1117/12.491288
Show Author Affiliations
Chi-Kuang Sun, National Taiwan Univ. (Taiwan)
Shi-Wei Chu, National Taiwan Univ. (Taiwan)
I-Hsiu Chen, National Taiwan Univ. (Taiwan)
Bai-Ling Lin, Institute of Molecular Biology (Taiwan)
Ping Chin Cheng, SUNY/Buffalo (United States)

Published in SPIE Proceedings Vol. 4620:
Multiphoton Microscopy in the Biomedical Sciences II
Ammasi Periasamy; Peter T. C. So, Editor(s)

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