
Proceedings Paper
Raman microscopy of cells: chemical imaging of apoptosisFormat | Member Price | Non-Member Price |
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Paper Abstract
Singular value decomposition was applied to the set of the
non-resonant Raman spectra, recorded during Raman imaging of the
single apoptotic cell. The basis vectors and the corresponding
singular values were assessed in terms of their statistical
significance. The noise-containing basis vectors were rejected
while keeping the meaningful ones. In this way the Raman images of
the apoptotic cell were successfully reconstructed from the
spectrally-filtered data matrix. The results are demonstrated on
the spatial distribution of the DNA, protein and phospholipids,
present in the fragments of the apoptotic cell.
Paper Details
Date Published: 10 July 2003
PDF: 8 pages
Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); doi: 10.1117/12.487609
Published in SPIE Proceedings Vol. 4963:
Multiphoton Microscopy in the Biomedical Sciences III
Ammasi Periasamy; Peter T. C. So, Editor(s)
PDF: 8 pages
Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); doi: 10.1117/12.487609
Show Author Affiliations
Cees Otto, Univ. of Twente (Netherlands)
Published in SPIE Proceedings Vol. 4963:
Multiphoton Microscopy in the Biomedical Sciences III
Ammasi Periasamy; Peter T. C. So, Editor(s)
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