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Proceedings Paper

Two-dimensional thermal analysis for freezing of plant and animal cells by high-speed microscopic IR camera
Author(s): Junko Morikawa; Toshimasa Hashimoto; Eita Hayakawa; Hideyuki Uemura
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Paper Abstract

Using a high speed IR camera for temperature sensor is a powerful new tool for thermal analysis in the cell scale biomaterials. In this study, we propose a new type of two-dimensional thermal analysis by means of a high speed IR camera with a microscopic lens, and applied it to the analysis of freezing of plant and animal cells. The latent heat on the freezing of super cooled onion epidermal cell was randomly observed by a unit cell size, one by one, under a cooling rate of 80degC/min with a spatial resolution of 7.5m. The freezing front of ice formation and the thermal diffusion behavior of generated latent heat were analyzed. As a result it was possible to determine simultaneously the intercellular/intracellular temperature distribution, the growing speed of freezing front in a single cell, and the thermal diffusion in the freezing process of living tissue. A new measuring system presented here will be significant in a transient process of biomaterials. A newly developed temperature wave methods for the measurement of in-plane thermal diffusivity was also applied to the cell systems.

Paper Details

Date Published: 1 April 2003
PDF: 6 pages
Proc. SPIE 5073, Thermosense XXV, (1 April 2003); doi: 10.1117/12.487047
Show Author Affiliations
Junko Morikawa, Tokyo Institute of Technology (Japan)
Toshimasa Hashimoto, Tokyo Institute of Technology (Japan)
Eita Hayakawa, Tokyo Institute of Technology (Japan)
Hideyuki Uemura, Seki Technotron Corp. (Japan)

Published in SPIE Proceedings Vol. 5073:
Thermosense XXV
K. Elliott Cramer; Xavier P. Maldague, Editor(s)

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