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Proceedings Paper

Detection of microbial cells and preliminary estimation of their physiological state by x-ray microanalysis
Author(s): Vladimir V. Sorokin; Andrei L. Mulyukin; Elena A. Vorobyova; Natalia E. Suzina; Vitalii I. Duda; Galina I. El-Registan
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Paper Abstract

The method of X-ray microanalysis was used for the detection of microbial cells among cell-like particles immediately in the samples of 170 thousand year old ancient Antarctic permafrost sediments. The X-ray spectra and the quantitative parameters of P, S, Ca and K contents in cell-like particles, as well as ratios Ca/K and P/S, were compared with the corresponding data obtained for microbial cells of various physiological states: vegetative cells -- resting forms -- non-viable micromummies. The absence of P and S peaks in X-ray spectra in the most of cell-like particles allowed us to regard them as non-living objects. Among other investigated cell-like particles we were able to find the resting forms of microorganisms with the increased intracellular level of Ca, high Ca/K ratio and low P/S ratio. So, X-ray microanalysis is a promising tool for a primary detection of microbial cells in situ and determination of their physiological state.

Paper Details

Date Published: 30 January 2003
PDF: 5 pages
Proc. SPIE 4939, Instruments, Methods, and Missions for Astrobiology VI, (30 January 2003);
Show Author Affiliations
Vladimir V. Sorokin, Institute of Microbiology (Russia)
Andrei L. Mulyukin, Institute of Microbiology (Russia)
Elena A. Vorobyova, Moscow State Univ. (Russia)
Natalia E. Suzina, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms (Russia)
Vitalii I. Duda, G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms (Russia)
Galina I. El-Registan, Institute of Microbiology (Russia)

Published in SPIE Proceedings Vol. 4939:
Instruments, Methods, and Missions for Astrobiology VI
Richard B. Hoover; Alexei Yu. Rozanov; Jere H. Lipps, Editor(s)

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