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Proceedings Paper

Multiparameter single-molecule fluorescence measurements of DNA intercalating fluorophores
Author(s): Benjamin P. Bowen; Jorg Enderlein; Neal W. T. Woodbury
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Paper Abstract

Experiments using single-molecules of TOTO-1 intercalated into dsDNA were performed to investigate the DNA sequence dependence on the fluorescence detectable with single-molecule fluorescence spectroscopy. Previous work has shown that there is a difference in the fluorescence lifetime when TOTO-1 is intercalated in poly-AT DNA or in poly-GC DNA. The fluorescence detected from single-molecules in this work for poly-GC and poly-AT DNA showed fluorescence lifetimes of 2.1 and 1.8 nsec, respectively. Analysis of the fluorescence intensity detected from single-molecules of TOTO-1 was performed by fluorescence cross-correlation spectroscopy. TOTO-1 is shown to spend large amounts of time in dark states. These dark states reduce the detectable fluorescence intensity to approximately 10 photons per millisecond on average.

Paper Details

Date Published: 19 June 2003
PDF: 5 pages
Proc. SPIE 4962, Manipulation and Analysis of Biomolecules, Cells, and Tissues, (19 June 2003); doi: 10.1117/12.486348
Show Author Affiliations
Benjamin P. Bowen, Arizona State Univ. (United States)
Jorg Enderlein, Forschungszentrum Julich (Germany)
Neal W. T. Woodbury, Arizona State Univ. (United States)

Published in SPIE Proceedings Vol. 4962:
Manipulation and Analysis of Biomolecules, Cells, and Tissues
Dan V. Nicolau; Joerg Enderlein; Robert C. Leif; Daniel L. Farkas, Editor(s)

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