Share Email Print

Proceedings Paper

SPECT imaging of pulmonary blood flow in a rat
Author(s): Christian Wietholt; Robert C. Molthen; Roger H. Johnson; Christopher A. Dawson; Anne V. Clough
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Small animal imaging is experiencing rapid development due to its importance in providing high-throughput phenotypic data for functional genomics studies. We have developed a single photon emission computed tomography (SPECT) system to image the pulmonary perfusion distribution in the rat. A standard gamma camera, equipped with a pinhole collimator, was used to acquire SPECT projection images at 40 sec/view of the rat thorax following injection of Tc99m labeled albumin that accumulated in the rat's lungs. A voxel-driven, ordered-subset expectation maximization reconstruction was implemented. Following SPECT imaging, the rat was imaged using micro-CT with Feldkamp conebeam reconstruction. The two reconstructed image volumes were fused to provide a structure/function image of the rat thorax. Reconstruction accuracy and performance were evaluated using numerical simulations and actual imaging of an experimental phantom consisting of Tc99m filled chambers with known diameters and count rates. Full-width half-maximum diameter measurement errors decreased with increasing chamber diameter, ranging from < 6% down to 0.1%. Errors in the ratio of count rate estimates between tubes were also diameter dependent but still relatively small. This preliminary study suggests that SPECT will be useful for imaging and quantifying the pulmonary blood flow distribution and the distribution of Tc99m labeled ligands in the lungs of small laboratory animals.

Paper Details

Date Published: 2 May 2003
PDF: 10 pages
Proc. SPIE 5031, Medical Imaging 2003: Physiology and Function: Methods, Systems, and Applications, (2 May 2003); doi: 10.1117/12.485802
Show Author Affiliations
Christian Wietholt, Marquette Univ. (United States)
Robert C. Molthen, Marquette Univ. (United States)
Medical College of Wisconsin (United States)
Roger H. Johnson, Marquette Univ. (United States)
Christopher A. Dawson, Marquette Univ. (United States)
Medical College of Wisconsin (United States)
Anne V. Clough, Marquette Univ. (United States)

Published in SPIE Proceedings Vol. 5031:
Medical Imaging 2003: Physiology and Function: Methods, Systems, and Applications
Anne V. Clough; Amir A. Amini, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?