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Proceedings Paper

Remote Raman spectroscopic imaging of human artery wall
Author(s): Darith Phat; Phat Ngoc Vuong; Pascal Plaza; Francis Cheilan; Nguyen Quy Dao
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Paper Abstract

Fourier-transform (FT) Raman spectroscopy using near-infrared (IR) excitation has been studied as a mean to diagnose pathology in human tissues by differentiating between the six types of atherosclerotic and healthy tissues. The importance of this technique lies in the fact that with minimal surgical invasion of the Raman scattering can be collected remotely through optical fibers with a near-IR FT Raman spectrometer. To date, 17 fresh autopsic human-aorta samples were studied in vitro using Nd:YAG laser and a power varying from 50 to 80 mW. Raman spectroscopy indicated a broad-continuum emission between 100 and 3500 cm-1 with Raman peaks of almost equal intensity at wavelengths of 1450 and 1659 cm-1 (R equals ratio; 0.96 +/- 0.06) for healthy intima. Fatty streaks (type I lesion), uncalcified atheromas (type II: lipid, fibrous, and mixed), ulcered lesions (type IIIu) and ulcero-calcified lesions (type IIIu+c, all with low Raman intensity, were found to have lower Raman intensity peak ratio values of 0.74 +/- 0.040, 0.79 +/- 0.022, 0.88 +/- 0.017, and 0.83 +/- 0.016 (p c) were characterized by very strong overall Raman intensity, very low peak ratio (0.57 +/- 0.062, p -1 allowing for precise diagnosis. This diagnostic information, which was based on intensity ratios (I1659/I1450), correlated well with histologic and biochemical compositions. These findings were not only crucial for the achievement of successful laser angioplasty but also promising for the in vivo studies of atherogenesis.

Paper Details

Date Published: 1 November 1991
PDF: 10 pages
Proc. SPIE 1525, Future Trends in Biomedical Applications of Lasers, (1 November 1991); doi: 10.1117/12.48228
Show Author Affiliations
Darith Phat, Ecole Centrale de Paris (France)
Phat Ngoc Vuong, Hopital Saint-Michel (France)
Pascal Plaza, Ecole Centrale de Paris (France)
Francis Cheilan, Hopital Saint-Michel (France)
Nguyen Quy Dao, Ecole Centrale de Paris (France)

Published in SPIE Proceedings Vol. 1525:
Future Trends in Biomedical Applications of Lasers
Lars Othar Svaasand, Editor(s)

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