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Proceedings Paper

Changes of ultraweak biochemiluminescence from germinating soybean in the wounding defense response
Author(s): Wenli Chen; Da Xing; Juan Wang
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Paper Abstract

Ultra-weak bio-chemiluminescence (UBC) from germinating soybean (Glycine max L. Merr) cotyledon under mechanical wounding was observed by a high sensitive single photon counter (SPC) device. SPC measuring results showed that the UBC intensity of wounding green cotyledon was very high firstly and reached to a stationary state after about 5 minutes. Wounding-induced emission could be suppressed by wounding treatment with sodium azide. While the intensity increase of the wounded etiolated soybean cotyledon was less than the green one. Deutenum oxide both amplified the emission intensity of green and etiolated. Singlet oxygen (102) is a main cause of the emission during the wounding phase. We suggested that the main process was probably that chioroplast and mitochondria damage at the wounded place and leaded to ROS generation, such as H202, 102 etc. The electronic excitation energy transfered from the excited molecules by ROS to Chl-a in the thylakoid membranes by way of relatively high quantum yields, so the intensity of UBC in the wounded soybean green cotyledon was obviously higher than in the wounded etiolated soybean cotyledon.

Paper Details

Date Published: 9 September 2002
PDF: 7 pages
Proc. SPIE 4920, Advanced Sensor Systems and Applications, (9 September 2002); doi: 10.1117/12.481946
Show Author Affiliations
Wenli Chen, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)
Juan Wang, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 4920:
Advanced Sensor Systems and Applications
Yun-Jiang Rao; Julian D. C. Jones; Hiroshi Naruse; Robert I. Chen, Editor(s)

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