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Proceedings Paper

Confocal scanning system with holographic notch filters used for gene-chip detection
Author(s): Tianchun Zhu; Ce Wang; Jianhong Wu; Minxue Tang; Yayi Zhu; Xiuzhou Feng; Linsen Chen
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Paper Abstract

Gene-chip as a very useful means of biotechnology has been applied to many research areas such as gene discovery, disease diagnosis and pharmacogenomics etc. , but commercially available gene-chip detection instruments are too expensive to be accepted by common labs. In this paper, a laser confocal scanning system with holographic notch filters used for simple gene-chip detection is presented. The system is mainly composed of an ordinary optical microscope, two holographic notch filters, a He-Ne laser, a digital controlled X-Y platform, a photodetector and a PC. The volume holographic notch filters were fabricated in our lab to substitute for the expensive narrow-band interference excitation filter and the dichroic filter in the conventional confocal detection system. The detection experiment was carried out on the gene-chips hybridized with cy5-labeled targets and the system sensitivity was determined by static measurement. The sensitivity is about 2x10-22mol/µm2, and the resolution is 10µm. The results indicate that the system can be used in primary gene-chip detection.

Paper Details

Date Published: 30 August 2002
PDF: 5 pages
Proc. SPIE 4924, Holography, Diffractive Optics, and Applications, (30 August 2002); doi: 10.1117/12.481497
Show Author Affiliations
Tianchun Zhu, Suzhou Univ. (China)
Ce Wang, Suzhou Univ. (China)
Jianhong Wu, Suzhou Univ. (China)
Minxue Tang, Suzhou Univ. (China)
Yayi Zhu, Suzhou Univ. (China)
Xiuzhou Feng, Suzhou Univ. (China)
Linsen Chen, Suzhou Univ. (China)

Published in SPIE Proceedings Vol. 4924:
Holography, Diffractive Optics, and Applications
Dahsiung Hsu; Jiabi Chen; Yunlong Sheng, Editor(s)

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