Share Email Print

Proceedings Paper

Optical property measurements of a novel type of upconverting reporter
Author(s): Xudong Xiao; Michael E. Herring; Jeanne Haushalter; Seonkyung Lee; Kostas S. Kalogerakis; Gregory W. Faris
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

We have recently developed a new type of reporter (upconverting chelate) for biomedical diagnostics. For this reporter, the light is absorbed and emitted by a lanthanide ion, rather than an organic molecule, as is the case for a typical fluorescent dye. These materials do not photobleach and have no autofluorescent background. We focus in this paper on neodymium ions complexed with the familiar chelating agents, EDTA, DPA, DTPA and DOTA. We have performed experimental measurements with one- and two-color laser light excitation for different chelate compounds. The samples are excited using two Nd:YAG-pumped dye laser systems that provide laser light near 587 nm and 800 nm. For one-color excitation, the emitted light depends quadratically on the incident laser power, as expected. Three strongly emitting lines are observed, located near 360 nm, 387 nm, and 417 nm. We observed more efficient upconversion in EDTA although the DPA chelates show comparable ground state absorbance. We have studied the influence of temporal delay between the two laser pulses and obtained the decay lifetime of the first intermediate state in the various chelated compounds.

Paper Details

Date Published: 12 September 2003
PDF: 7 pages
Proc. SPIE 4967, Genetically Engineered and Optical Probes for Biomedical Applications, (12 September 2003); doi: 10.1117/12.479841
Show Author Affiliations
Xudong Xiao, SRI International (United States)
Michael E. Herring, SRI International (United States)
Jeanne Haushalter, SRI International (United States)
Seonkyung Lee, SRI International (United States)
Kostas S. Kalogerakis, SRI International (United States)
Gregory W. Faris, SRI International (United States)

Published in SPIE Proceedings Vol. 4967:
Genetically Engineered and Optical Probes for Biomedical Applications
Darryl J. Bornhop; Alexander P. Savitsky; Ramesh Raghavachari; Samuel I. Achilefu, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?