Share Email Print

Proceedings Paper

Possible mechanisms for optical clearing of whole blood by dextrans
Author(s): Ruikang K. Wang; Xiangqun Xu; James B. Elder; Valery V. Tuchin
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

The purpose of the present study is to investigate systematically the mechanisms of alterations in the optical properties of whole blood immersed in the biocompatible agent dextrans, and to define the optimal concentration of dextrans required for blood optical clearing in order to enhance the capability of light penetration depth for optical imaging applications. In the experiments, dextrans with different molecular weights and various concentrations were employed and investigated by the use of the optical coherence tomography technique. Changes in light attenuation, refractive index and aggregation properties of blood immersed in dextrans were studied. It was concluded from the results that the mechanisms for blood optical clearing are the characteristics of the types of dextrans employed, their concentrations, and the application stages. Among the substances applied, DX500 at a concentration at 0.5gdl-1 gives the best result in improving light penetration depth through the blood. The increase of light transmission at the beginning of the addition of dextrans is mainly attributed to refractive index matching between the scattering centers and the ground matter. Thereafter, the transmission change is probably due to dextran-induced aggregation-disaggregation effect. Overall, light scattering in the blood could be effectively reduced by the application of dextrans. It represents a promising approach to increasing the imaging depth for in vivo optical imaging of biological tissue, for example optical coherence tomography.

Paper Details

Date Published: 23 July 2003
PDF: 11 pages
Proc. SPIE 4965, Optical Diagnostics and Sensing in Biomedicine III, (23 July 2003); doi: 10.1117/12.479191
Show Author Affiliations
Ruikang K. Wang, Cranfield Univ. (United Kingdom)
Tianjin Univ. (China)
Xiangqun Xu, Cranfield Univ. (United Kingdom)
James B. Elder, Keele Univ. School of Medicine (United Kingdom)
Valery V. Tuchin, Saratov State Univ. (Russia)

Published in SPIE Proceedings Vol. 4965:
Optical Diagnostics and Sensing in Biomedicine III
Alexander V. Priezzhev; Gerard L. Cote, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?