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Proceedings Paper

In vivo cytochrome P450 drug metabolizing enzyme characterization using surface-enhanced Raman spectroscopy
Author(s): Yanfang Li; Kenneth A. Bachmann; Brent D. Cameron
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Paper Abstract

The development of a rapid, inexpensive, and accurate in vivo phenotyping methodology for characterizing drug-metabolizing phenotypes with reference to the cytochrome P450 (CYP450) enzymes would be very beneficial. In terms of application, in the wake of the human genome project, considerable interest is focused on the development of new drugs whose uses will be tailored to specific genetic polymorphisms, and on the individualization of dosing regimens that are also tailored to meet individual patient needs depending upon genotype. In this investigation, chemical probes for CYP450 enzymes were characterized and identified with Raman spectroscopy. Furthermore, gold-based metal colloid clusters were utilized to generate surface enhanced Raman spectra for each of the chemical probes. Results will be presented demonstrating the ability of SERS to identify minute quantities of these probes on the order needed for in vivo application.

Paper Details

Date Published: 12 September 2003
PDF: 6 pages
Proc. SPIE 4967, Genetically Engineered and Optical Probes for Biomedical Applications, (12 September 2003); doi: 10.1117/12.478394
Show Author Affiliations
Yanfang Li, Univ. of Toledo (United States)
Kenneth A. Bachmann, Univ. of Toledo (United States)
Brent D. Cameron, Univ. of Toledo (United States)

Published in SPIE Proceedings Vol. 4967:
Genetically Engineered and Optical Probes for Biomedical Applications
Darryl J. Bornhop; Alexander P. Savitsky; Ramesh Raghavachari; Samuel I. Achilefu, Editor(s)

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