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Proceedings Paper

Determination of subsurface tumor localization in animal models with near-infrared (NIR) fluorescence imaging
Author(s): Yu Chen; Dana M. Blessington; Zhihong Zhang; Qian Liu; Lanlan Zhou; Chenpeng Mu; Xavier Intes; Samuel I. Achilefu; Hui Li; Min Z. Zhang; Gang Zheng; Britton Chance
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Paper Abstract

We have developed a novel imaging system for determining the localization of tumors labeled by fluorescent contrast agents and embedded several centimeters inside the highly scattered medium. This frequency-domain system utilizes the phased cancellation configuration with a goniometric probe. The instrumentation performance on the phantom test can detect 3 mm diameter sphere filled with 1 nM fluorescent dye, Indocyanine Green (ICG), and 3 cm deep inside the scattering medium with similar optical properties as human breast tissue within a 1 mm localization confidence. Mouse tumor model immersed in appropriate scattering/absorbing medium is used for animal test. Intra-tumor injection of ICG demonstrates the localization of the tumor (5 mm in diameter) submerged 3 cm deep inside the highly scattered medium with 2 mm position error. Results with NIR804-D-Glucosamide on the AR42J tumor bearing nude mouse are also presented with 3 mm localization error. The accuracy of the localization suggest that this system would be helpful to guide the clinical fine-needle biopsy for early breast cancer detection.

Paper Details

Date Published: 29 July 2003
PDF: 8 pages
Proc. SPIE 4955, Optical Tomography and Spectroscopy of Tissue V, (29 July 2003); doi: 10.1117/12.478187
Show Author Affiliations
Yu Chen, Univ. of Pennsylvania (United States)
Dana M. Blessington, Univ. of Pennsylvania (United States)
Zhihong Zhang, Univ. of Pennsylvania (United States)
Huazhong Univ. of Science and Technology (China)
Qian Liu, Univ. of Pennsylvania (United States)
Huazhong Univ. of Science and Technology (United States)
Lanlan Zhou, Univ. of Pennsylvania (United States)
Chenpeng Mu, Univ. of Pennsylvania (United States)
Xavier Intes, Univ. of Pennsylvania (United States)
Samuel I. Achilefu, Washington Univ. School of Medicine (United States)
Hui Li, Univ. of Pennsylvania (United States)
Min Z. Zhang, Univ. of Pennsylvania (United States)
Gang Zheng, Univ. of Pennsylvania (United States)
Britton Chance, Univ. of Pennsylvania (United States)

Published in SPIE Proceedings Vol. 4955:
Optical Tomography and Spectroscopy of Tissue V
Britton Chance; Robert R. Alfano; Bruce J. Tromberg; Mamoru Tamura; Eva M. Sevick-Muraca, Editor(s)

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