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Proceedings Paper

Resonant mass biosensor for ultrasensitive detection of bacterial cells
Author(s): Amit Gupta; Demir Akin; Rashid Bashir
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Paper Abstract

This paper describes a surface micromachined cantilever beam based oscillator detector for biological applications. This study used a novel microfabrication technique of merged epitaxial lateral overgrowth (MELO) and chemical mechanical polishing (CMP) to fabricate thin, low stress, single-crystal silicon cantilever beams. Vibration spectra of the cantilever beams, excited by thermal and ambient noise, was measured in air using a Digital Instrument Dimension 3100 Series scanning probe microscope (SPM). The cantilever beams were calibrated by obtaining the spring constant using the added-mass method. The sensors were used to detect the presence of Listeria innocua bacteria by applying increasing concentration of bacteria suspension on the same cantilever beam and measuring the resonant frequency changes in air. Cantilever beams were also used to detect the mass of the adsorbed antibodies and used to show selective capture of bacterial cells. The results indicate that the developed biosensor is capable of rapid and ultra-sensitive detection of bacteria and promises significant potential for enhancement of microbiological research and diagnostics.

Paper Details

Date Published: 17 January 2003
PDF: 7 pages
Proc. SPIE 4982, Microfluidics, BioMEMS, and Medical Microsystems, (17 January 2003); doi: 10.1117/12.478147
Show Author Affiliations
Amit Gupta, Purdue Univ. (United States)
Demir Akin, Purdue Univ. (United States)
Rashid Bashir, Purdue Univ. (United States)

Published in SPIE Proceedings Vol. 4982:
Microfluidics, BioMEMS, and Medical Microsystems
Holger Becker; Peter Woias, Editor(s)

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