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Proceedings Paper

Use of molecular beacons for the detection of bacteria in microfluidic devices
Author(s): Chuanwu Xi; Stephen A. Boppart; Lutgarde Raskin
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Paper Abstract

We present the use of DNA and peptide nucleic acid (PNA) molecular beacons (MBs) as sensitive indicators in microfluidic bioMEM devices. DNA and PNA MBs can be used to quantitatively study hybridization kinetics in real time in a polydimethyl siloxane (PDMS) microfluidic device. PNA MBs perform better than DNA MBs for the study of hybridization kinetics of rRNA targets in real time in microfluidic channels. We also demonstrate the use of PNA MBs for fast detection of bacterial cells in microfluidic channels. Using PNA MBs as detection probes will enable us to develop an integrated biosensor for the rapid and on-site detection and quantification of microbial pathogens in environmental and clinical samples.

Paper Details

Date Published: 17 January 2003
PDF: 8 pages
Proc. SPIE 4982, Microfluidics, BioMEMS, and Medical Microsystems, (17 January 2003); doi: 10.1117/12.478143
Show Author Affiliations
Chuanwu Xi, Univ. of Illinois/Urbana-Champaign (United States)
Stephen A. Boppart, Univ. of Illinois/Urbana-Champaign (United States)
Lutgarde Raskin, Univ. of Illinois/Urbana-Champaign (United States)

Published in SPIE Proceedings Vol. 4982:
Microfluidics, BioMEMS, and Medical Microsystems
Holger Becker; Peter Woias, Editor(s)

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