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Proceedings Paper

Monitoring enzymatic reactions with in situ sensors
Author(s): Ian T. Young; V. Iordanov; Arthur Kroon; Heidi R. C. Dietrich; R. Moerman; L. Richard van den Doel; G.W. K. van Dedem; Andre Bossche; Bonnie L. Gray; Lina Sarro; Piet W. Verbeek; Lucas J. van Vliet
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Paper Abstract

In previous publications and presentations we have described our construction of a laboratory-on-a-chip based on nanoliter capacity wells etched in silicon. We have described methods for dispensing reagents as well as samples, for preventing evaporation, for embedding electronics in each well to measure fluid volume per well in real-time, and for monitoring the production or consumption of NADH in enzyme-catalyzed reactions such as those found in the glycolytic pathway of yeast. In this paper we describe the use of light sensors (photodiodes) in each well to measure both fluorescence (such as that evidenced in NADH) as well as bioluminescence (such as evidenced in ATP assays). We show that our detection limit for NADH fluorescence in 100 μM and for ATP/luciferase bioluminescence is 2.4 μM.

Paper Details

Date Published: 18 July 2003
PDF: 7 pages
Proc. SPIE 4966, Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis, (18 July 2003); doi: 10.1117/12.478088
Show Author Affiliations
Ian T. Young, Delft Univ. of Technology (Netherlands)
V. Iordanov, Delft Univ. of Technology (Netherlands)
Arthur Kroon, Delft Univ. of Technology (Netherlands)
Heidi R. C. Dietrich, Delft Univ. of Technology (Netherlands)
R. Moerman, Delft Univ. of Technology (Netherlands)
L. Richard van den Doel, Delft Univ. of Technology (Netherlands)
G.W. K. van Dedem, Delft Univ. of Technology (Netherlands)
Andre Bossche, Delft Univ. of Technology (Netherlands)
Bonnie L. Gray, Delft Univ. of Technology (Netherlands)
Lina Sarro, Technische Univ. Delft (Netherlands)
Piet W. Verbeek, Delft Univ. of Technology (Netherlands)
Lucas J. van Vliet, Delft Univ. of Technology (Netherlands)

Published in SPIE Proceedings Vol. 4966:
Microarrays and Combinatorial Technologies for Biomedical Applications: Design, Fabrication, and Analysis
Dan V. Nicolau; Ramesh Raghavachari, Editor(s)

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