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Proceedings Paper

Effects of index-mismatch-induced spherical aberration on two-photon imaging in skin and tissue-like constructs
Author(s): Chih-kuan Tung; Tsung-Kai Chiu; Wen Lo; Po-Hsiang Wang; Sun-Jan Lin; Shiou-Hwa Jee; Chen-Yuan Dong
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Paper Abstract

Index mismatch induced spherical aberration is studied by comparing imaging ability of different immersion objectives in both uniformly fluorescent solutions and fluorescently labeled skin samples using scanning two-photon fluorescence microscopy. We investigated the performances of the objectives (air, water, glycerin, and oil immersion) by measuring the fluorescence profiles at different depths. In homogeneous fluorescent samples, we found that immersion medium with compatible refractive index as the samples yields better results, and small differences in refractive indices did not cause noticeable effects. Similar results were found in skin samples. Except for the air objective, we found that the choice of immersion medium did not have significant effects for in-depth imaging in the fluorescent solutions or skin samples.

Paper Details

Date Published: 10 July 2003
PDF: 10 pages
Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); doi: 10.1117/12.478026
Show Author Affiliations
Chih-kuan Tung, National Taiwan Univ. (Taiwan)
Tsung-Kai Chiu, National Taiwan Univ. (Taiwan)
Wen Lo, National Taiwan Univ. (Taiwan)
Po-Hsiang Wang, National Taiwan Univ. (Taiwan)
Sun-Jan Lin, National Taiwan Univ. Hospital (Taiwan)
Shiou-Hwa Jee, National Taiwan Univ. Hospital (Taiwan)
National Taiwan Univ. College of Medicine (Taiwan)
Chen-Yuan Dong, National Taiwan Univ. (Taiwan)

Published in SPIE Proceedings Vol. 4963:
Multiphoton Microscopy in the Biomedical Sciences III
Ammasi Periasamy; Peter T. C. So, Editor(s)

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