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Proceedings Paper

High-resolution imaging of biological samples
Author(s): Ulrike Tauer; Reiner Rygiel; Martin Hoppe
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Paper Abstract

Confocal laser scanning microscopy is used in many different fields of research nowadays. Therefore, high spatial resolution is required as well as high temporal resolution. Further, the quality of the resulting images has to be considered. Doing imaging with laser scanning microscopes, the balance between spatial resolution, speed and signal-to-noise ratio has to be defined for every specimen and experiment individually. Special adaptations to standard laser scanning microscopes improve the efficiency of the Leica TCS SP2 and Leica TCS SP2 RS for high-resolution low-noise imaging. Here, we want to report from the acousto-optical beam splitter, the resonant K-scanner and new objectives.

Paper Details

Date Published: 9 July 2003
PDF: 6 pages
Proc. SPIE 4964, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X, (9 July 2003); doi: 10.1117/12.478009
Show Author Affiliations
Ulrike Tauer, Leica Microsystems Heidelberg GmbH (Germany)
Reiner Rygiel, Leica Microsystems Heidelberg GmbH (Germany)
Martin Hoppe, Leica Microsystems Heidelberg GmbH (Germany)

Published in SPIE Proceedings Vol. 4964:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing X
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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