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Proceedings Paper

Digital in-line holography with numerical reconstruction: 4D tracking of microstructures and organisms
Author(s): Hans Jurgen Kreuzer; M. H. Jericho; I. A. Meinertzhagen; W. Xu
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Paper Abstract

Using holographic microscopy we have been able to visualize submicron-sized. bacteria in-vivo. A simple holographic method enables us to capture as a single data set the trajectories of micron size objects suspended in water. By subtracting consecutive holograms of a particle suspension and then adding these difference holograms, a final data set is constructed that contains the time evolution of the particle trajectories free from spurious background interference effects. Temporal and spatial resolution at the sub-second and sub-micron levels can easily be achieved. The method is illustrated by recording the motion in 3-D of 5μm diameter latex spheres subject to gravity and electrostatic fields to visualize their micro-fluidic flow. Another example is the 3-D motion of a collection of algae, protozoa and bacteria in water.

Paper Details

Date Published: 4 June 2003
PDF: 8 pages
Proc. SPIE 5005, Practical Holography XVII and Holographic Materials IX, (4 June 2003); doi: 10.1117/12.476875
Show Author Affiliations
Hans Jurgen Kreuzer, Dalhousie Univ. (Canada)
M. H. Jericho, Dalhousie Univ. (Canada)
I. A. Meinertzhagen, Dalhousie Univ. (Canada)
W. Xu, Dalhousie Univ. (Canada)

Published in SPIE Proceedings Vol. 5005:
Practical Holography XVII and Holographic Materials IX
Tung H. Jeong; Sylvia H. Stevenson, Editor(s)

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