The aim of this study is to investigate the feasability of using a needle-free injection gun to inject highly viscous glycerol into porcine skin for the purpose of optical skin clearing. In this study a needle-free injection device DERMO-JET by Robbins Instruments was used to inject glycerol in 100%, 50% and 25% concentration into freshly excised porcine skin. The injection sites were imaged using optical coherence tomography (OCT) amplitude data to determine injection depth. Similar experiments were conducted involving the same device for injection of 100%, 50% and 25% glycerol-water mixtures with trace amounts of rhodamine. Injected tissue was rapidly frozen and sectioned with a keratome to reveal the mixture dispersion with a fluorescence microscope.

Date Published: 12 September 2003
PDF: 7 pages
Proc. SPIE 4949, Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII, (12 September 2003); doi: 10.1117/12.476147

Published in SPIE Proceedings Vol. 4949:
Lasers in Surgery: Advanced Characterization, Therapeutics, and Systems XIII
Eugene A. Trowers M.D.; Lawrence S. Bass M.D.; Udayan K. Shah M.D.; Reza S. Malek M.D.; David S. Robinson M.D.; Kenton W. Gregory M.D.; Lawrence S. Bass M.D.; Abraham Katzir; Nikiforos Kollias; Hans-Dieter Reidenbach; Brian Jet-Fei Wong M.D.; Timothy A. Woodward M.D.; Werner T.W. de Riese; Keith D. Paulsen, Editor(s)