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Proceedings Paper

Single-cell analysis on a microchip platform using optical tweezers and optical scissors
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Paper Abstract

Microfluidic devices, or "lab-on-a-chip" systems for single cell analysis represent a new field of micro-total analysis systems (μTAS) that could not only perform a task quicker, and more accurately than conventional methods, but could also incorporate additional tools to the study of biological variability in a population by allowing researchers to directly examine the contents of a multitude of single cells from the population under study. Realizing such a device, presents several engineering challenges to the fields of micro-machining, micromanipulation and analytical bio-chemistry. The device needs to be able to accurately and efficiently select, manipulate and analyze volumes represented by a single cell without diluting the contents. For this purpose, optical tweezers and scissors were implemented to select single cells on a microchip, bring the cell to a desired location, and lyse the cell using the optical scissors. Channels were engineered in the device using a molecular fluorine (F2) laser. Each channel’s cross-section is approximately the size of an individual cell (10μm wide and deep). This paper, describes the manipulation of cells on a microchip using optical tweezers and the injection of the cellular contents by optical scissors from a single cell into 10μm channels.

Paper Details

Date Published: 17 January 2003
PDF: 9 pages
Proc. SPIE 4982, Microfluidics, BioMEMS, and Medical Microsystems, (17 January 2003); doi: 10.1117/12.472892
Show Author Affiliations
Nigel R. Munce, Univ. of Toronto (Canada)
Jianzhao Li, Univ. of Toronto (Canada)
Peter R. Herman, Univ. of Toronto (Canada)
Lothar D. Lilge, Univ. of Toronto (Canada)

Published in SPIE Proceedings Vol. 4982:
Microfluidics, BioMEMS, and Medical Microsystems
Holger Becker; Peter Woias, Editor(s)

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