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Proceedings Paper

Hyper-Rayleigh scattering (HRS) as a mechanism for biphotonic fluorescence excitation in microscopy
Author(s): Melville R. V. Sahyun; Matthew Goertz
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Paper Abstract

We have observed fluorescence from Coumarin 334 in solution using light of twice an actinic wavelength for excitation. The effect is demonstrated to be biphotonic, but is observed at optical powers below those required for resonant biphotonic excitation, and with an incoherent source. We infer operation of HRS. By extrapolation we propose that this effect should contribute to fluorescence under usual conditions for multiphoton fluorescence microscopy, and that it might be exploited in fluorescence microscopy under conditions where usual mechanisms are inapplicable.

Paper Details

Date Published: 10 July 2003
PDF: 6 pages
Proc. SPIE 4963, Multiphoton Microscopy in the Biomedical Sciences III, (10 July 2003); doi: 10.1117/12.472868
Show Author Affiliations
Melville R. V. Sahyun, Univ. of Wisconsin/Eau Claire (United States)
Matthew Goertz, Univ. of Wisconsin/Eau Claire (United States)

Published in SPIE Proceedings Vol. 4963:
Multiphoton Microscopy in the Biomedical Sciences III
Ammasi Periasamy; Peter T. C. So, Editor(s)

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