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Proceedings Paper

Single-cell manipulation and fluorescence detection in benchtop flow cytometry system with disposable plastic microfluidic chip
Author(s): Jung Kyung Kim; Hyunwoo Bang; Seok Chung; Alexey Dan Chin-Yu; Chanil Chung; Han Sang Jo; Jung Yul Yoo; Jun Keun Chang
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Paper Abstract

Flow cytometry and microfluidic system are versatile tools to study the functional genomics at the single-cell level in the postgenomic era. We have developed a microfluidics-based benchtop flow cytometry system incorporating a disposable plastic microchip and low-power diode lasers. The plastic microfluidic chip is designed and fabricated using soft lithography which enables the development of inexpensive and flexible miniaturized fluidic devices. The microchip contains the hydrodyamic focusing chamber where the sample and sheath fluids are driven by a pressure difference. The performance of the combined fluidics and optics is studied systematically to evaluate the detection accuracy and efficiency of our flow cytometry system. The interactions of the biological particles with surrounding squeezed flow and focused laser beam are investigated to optimize the design of the microchannel network as well as the optical characteristics of the instrument for efficient single-cell manipulation and detection.

Paper Details

Date Published: 17 January 2003
PDF: 13 pages
Proc. SPIE 4982, Microfluidics, BioMEMS, and Medical Microsystems, (17 January 2003); doi: 10.1117/12.472741
Show Author Affiliations
Jung Kyung Kim, Digital Bio Technology Co., Ltd. (South Korea)
Hyunwoo Bang, Seoul National Univ. (South Korea)
Seok Chung, Digital Bio Technology Co., Ltd. (South Korea)
Alexey Dan Chin-Yu, Digital Bio Technology Co., Ltd. (South Korea)
Chanil Chung, Digital Bio Technology Co., Ltd. (South Korea)
Han Sang Jo, Digital Bio Technology Co., Ltd. (South Korea)
Jung Yul Yoo, Seoul National Univ. (South Korea)
Jun Keun Chang, Digital Bio Technology Co., Ltd. (South Korea)
Seoul National Univ. (South Korea)

Published in SPIE Proceedings Vol. 4982:
Microfluidics, BioMEMS, and Medical Microsystems
Holger Becker; Peter Woias, Editor(s)

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