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Proceedings Paper

Subcellular calcium signaling in cardiac cells revealed with fast two-dimensional confocal imaging
Author(s): Lothar A. Blatter M.D.; Katherine A. Sheehan; Jens Kockskaemper
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Paper Abstract

Excitation-contraction (e-c) coupling in atrial myocytes was studied with fast confocal microscopy and simultaneous Ca2+-current measurements. Cat atrial myocytes lack transverse tubules and contain junctional (j-SR) and non- junctional SR (nj-SR) which both ave ryanodine receptor Ca2+ release channels. ICa triggered Ca2+ release from discrete peripheral j-SR release sites, which then fused into a peripheral 'ring' of elevated (Ca2+)i, followed by propagation to the center and contraction. J-SR Ca2+ release could be terminated instantaneously by interrupting ICa, whereas nj-SR Ca2+ release continued, once initiated, even after ICa and j-SR Ca2+ release was terminated. Resting myocytes exhibited spontaneous Ca2+ release events including Ca2+ release sites. In summary, during e-c coupling in atrial cells the elevation of (Ca2+)i begins with j-SR Ca2+ release under the tight control of ICa. The peripheral increase of (Ca2+)i subsequently activates adjacent nj-SR resulting in centripetal propagation of activation via CICR.

Paper Details

Date Published: 21 June 2002
PDF: 11 pages
Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); doi: 10.1117/12.472112
Show Author Affiliations
Lothar A. Blatter M.D., Loyola Univ. (United States)
Katherine A. Sheehan, Loyola Univ. (United States)
Jens Kockskaemper, Loyola Univ. (United States)

Published in SPIE Proceedings Vol. 4626:
Biomedical Nanotechnology Architectures and Applications
Raymond P. Mariella Jr.; Michelle Palmer; Darryl J. Bornhop; Darryl J. Bornhop; Ramesh Raghavachari; Shuming Nie; Ramesh Raghavachari; Catherine J. Murphy; David A. Dunn; David A. Dunn; Raymond P. Mariella Jr.; Catherine J. Murphy; Dan V. Nicolau; Shuming Nie; Michelle Palmer; Ramesh Raghavachari, Editor(s)

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