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Proceedings Paper

Low-cost and high-throughput multichannel capillary electrophoresis (MCCE) system for DNA analysis
Author(s): Varoujan David Amirkhanian; Ming-Sun Liu
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Paper Abstract

In this paper we present the innovative use of an inexpensive Multi-Channel Capillary-based Electrophoresis (MCCE) system in combination with disposable separation cartridge for routine analysis of DNA fragments. The proposed multi-channel system s base don a novel multiplexed fluorescence detection technology, which provides a rapid and unique solution for DNA analysis. Presently the Capillary Electrophoresis (CE) based technology used for DNA analysis, rely on gas discharge UV-visible lamps or lasers as light sources that are bulky, expensive, and difficult to couple one's light output into optical fibers, for miniaturization of the optical detection system. The light sources hinder the development of small sized, high- throughput and cost-effective genomics instrument. Whereas, the proposed instrument with solid-state light sources and non-moving detection micro-optics, and re-usable cartridge containing multiple separation channels, provide a cost- effective solution for a robust CE instrument. Furthermore, the simplified operation of the MCCE instrument will drastically reduce the cost of DNA analysis, and possibly will be the instrument of choice for forensic DNA and molecular diagnostics applications in the near future.

Paper Details

Date Published: 21 June 2002
PDF: 9 pages
Proc. SPIE 4626, Biomedical Nanotechnology Architectures and Applications, (21 June 2002); doi: 10.1117/12.472087
Show Author Affiliations
Varoujan David Amirkhanian, BioCal Technology, Inc. (United States)
Ming-Sun Liu, BioCal Technology, Inc. (United States)

Published in SPIE Proceedings Vol. 4626:
Biomedical Nanotechnology Architectures and Applications
Raymond P. Mariella Jr.; Michelle Palmer; Darryl J. Bornhop; Darryl J. Bornhop; Ramesh Raghavachari; Shuming Nie; Ramesh Raghavachari; Catherine J. Murphy; David A. Dunn; David A. Dunn; Raymond P. Mariella Jr.; Catherine J. Murphy; Dan V. Nicolau; Shuming Nie; Michelle Palmer; Ramesh Raghavachari, Editor(s)

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