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Proceedings Paper

Recent advances on Coherent Anti-Strokes Raman scattering (CARS) microscopy
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Paper Abstract

Recent advances in CARS microscopy provided exciting possibilities for imaging living cells with high sensitivity, high 3D spatial resolution and chemical specificity based on vibrational spectroscopy. Epi-detection avoids the forward-going water signal and allows imaging of small features inside cells. Polarization-sensitive detection improves the vibrational contrast in CARS microscopy via suppression of the non-resonant background. High-speed CARS imaging of living cells is realized by raster scanning two collinearly overlapped near infrared picosecond laser beams. Vibrational mapping of lipids and proteins inside living cells is achieved. CARS imaging of unstained cells during mitosis and apoptosis is carried out. Incorporation of CARS microscopy with the photon correlation technique allows probing diffusion dynamics of 50-nm beads with chemical selectivity.

Paper Details

Date Published: 17 June 2002
PDF: 11 pages
Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); doi: 10.1117/12.470697
Show Author Affiliations
Ji-xin Cheng, Harvard Univ. (United States)
Eric O. Potma, Harvard Univ. (United States)
Sunney Xie, Harvard Univ. (United States)

Published in SPIE Proceedings Vol. 4620:
Multiphoton Microscopy in the Biomedical Sciences II
Ammasi Periasamy; Peter T. C. So, Editor(s)

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