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Proceedings Paper

Multiphoton microscopy of antigen presenting cells in experimental cancer therapies
Author(s): Simon C. Watkins; Glenn D. Papworth; Lori A. Spencer; Adriana T. Larregina; Holger Hackstein
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Paper Abstract

The absence of effective conventional therapy for most cancer patients justifies the application of novel, experimental approaches. One alternative to conventional cytotoxic agents is a more defined molecular approach for cancer immune treatment; promotion of the immune system specifically to target and eliminate tumor cells on the basis of expression of tumor-associated antigens (TAA). TAA could be presented to T-cells by professional antigen-presenting cells (APC) that generate a more efficient and effective anti-tumor immune response. In fact, it has been well documented that dendritic cells, the most immunologically potent APC, are capable of recognizing, processing and presenting TAA, in turn initiating a specific antitumor immune response. Results from several laboratories and clinical trials suggested significant but still limited efficacy of TAA-pulsed dendritic cells administered to tumor-bearing hosts. Following such delivery, it is fundamentally necessary to dynamically assess cell abundance within the microenvironment of the tumor in the presence of the appropriate therapeutic agent. Multiphoton microscopy was used to assess the trafficking of pulsed dendritic cells and other APC in skin, lymph nodes and brain of several animal tumor models, following different routes of administration.

Paper Details

Date Published: 17 June 2002
PDF: 7 pages
Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); doi: 10.1117/12.470684
Show Author Affiliations
Simon C. Watkins, Univ. of Pittsburgh (United States)
Glenn D. Papworth, Univ. of Pittsburgh (United States)
Lori A. Spencer, Univ. of Pittsburgh (United States)
Adriana T. Larregina, Univ. of Pittsburgh (United States)
Holger Hackstein, Univ. of Pittsburgh (United States)

Published in SPIE Proceedings Vol. 4620:
Multiphoton Microscopy in the Biomedical Sciences II
Ammasi Periasamy; Peter T. C. So, Editor(s)

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