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Proceedings Paper

Suitable microscope objectives for multiphoton digital imaging
Author(s): Gerald S. Benham; Stanley Schwartz
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Paper Abstract

The proper choice of an objective lens can have a profound effect on multiphoton microscopy. Multiphoton requires that the objective have adequate transmission in the IR range. This technology has a requisite to image the specimen near the slide surface next to the cover glass and to of 200 microns or more. Fluor and Plan Apochromat objectives. All lenses suffer from aberrations, which can prevent imaging an exact replica of the object. Most objectives could be used for multiphoton imaging;; however lenses should be selected from Plan Fluors, Plan Apochromats, and Super (S) Fluors. Highly corrected water immersion objectives (WI) permit deep penetration within aqueous environments and living tissues, beyond that of oil immersion objectives. Spectral transmission data is presented for Nikon CFI60 various objective lenses with high Nas, long working distances, and maximum aberration correction.

Paper Details

Date Published: 17 June 2002
PDF: 12 pages
Proc. SPIE 4620, Multiphoton Microscopy in the Biomedical Sciences II, (17 June 2002); doi: 10.1117/12.470673
Show Author Affiliations
Gerald S. Benham, Nikon Instruments Inc. (United States)
Stanley Schwartz, Nikon Instruments Inc. (United States)

Published in SPIE Proceedings Vol. 4620:
Multiphoton Microscopy in the Biomedical Sciences II
Ammasi Periasamy; Peter T. C. So, Editor(s)

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