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Proceedings Paper

Optical coherence imaging of rat tumor spheroids
Author(s): Ping Yu; M. Mustata; William Robert Headley; David D. Nolte; J. J. Turek; Paul M. W. French
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Paper Abstract

We report the first results of optical coherence imaging (OCI) of rat tumor spheroids. OCI is a full-frame variant of optical coherence tomography (OCT). The coherent image spatially modulates a high-sensitivity dynamic holographic film composed of a photorefractive quantum well (PRQW). Full-frame readout out of the hologram is observed in real time on a video camera. This system may be considered generally as a video camera with a coherence filter on the lens. Tumor spheroids are small (100-1000 m) balls of tumor cells that are cultured in vitro. Larger spheroids have increasingly complex inner structure. Necrosis and calcification form and expand, reminiscent of structure in malignant cysts in human tumors. In addition, rafts of tumor cells become separated by fluid-filled voids. These features are within the resolution limit of the OCI system, and produce highly structured coherent images.

Paper Details

Date Published: 14 June 2002
PDF: 9 pages
Proc. SPIE 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI, (14 June 2002); doi: 10.1117/12.470485
Show Author Affiliations
Ping Yu, Purdue Univ. (United States)
M. Mustata, Purdue Univ. (United States)
William Robert Headley, Purdue Univ. (United States)
David D. Nolte, Purdue Univ. (United States)
J. J. Turek, Purdue Univ. (United States)
Paul M. W. French, Imperial College of Science, Technology, and Medicine (United Kingdom)

Published in SPIE Proceedings Vol. 4619:
Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI
Valery V. Tuchin; Joseph A. Izatt; James G. Fujimoto, Editor(s)

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