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Proceedings Paper

Murine myocardium OCT imaging with a blood substitute
Author(s): Jeehyun Kim; Joseph W. Villard; Ho Lee; Marc D. Feldman; Thomas E. Milner
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Paper Abstract

Imaging of the in vivo murine myocardium using optical coherence tomography (OCT) is described. Application of conventional techniques (e.g. MRI, Ultrasound imaging) for imaging the murine myocardium is problematic because the wall thickness is less than 1.5mm (20g mouse), and the heart rate can be as high as six-hundred beats per minute. To acquire a real-time image of the murine myocardium, OCT can provide sufficient spatial resolution (10 micrometers ) and imaging speed (1000 A-Scans/s). Strong light scattering by blood in the heart causes significant light attenuation making delineation of the endocardium-chamber boundary problematic. By replacing whole blood in the mouse with an artificial blood substitute we demonstrate significant reduction of light scattering in the murine myocardium. The results indicate a significant reduction in light scattering as whole blood hematocrit is diminished below 5%. To measure thickness change of the myocardium during one cycle, a myocardium edge detection algorithm is developed and demonstrated.

Paper Details

Date Published: 14 June 2002
PDF: 6 pages
Proc. SPIE 4619, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI, (14 June 2002); doi: 10.1117/12.470479
Show Author Affiliations
Jeehyun Kim, Univ. of Texas at Austin (United States)
Joseph W. Villard, Univ. of Texas at Austin (United States)
Ho Lee, Univ. of Texas at Austin (United States)
Marc D. Feldman, Univ. of Texas Health Science Ctr. (United States)
Thomas E. Milner, Univ. of Texas at Austin (United States)

Published in SPIE Proceedings Vol. 4619:
Coherence Domain Optical Methods in Biomedical Science and Clinical Applications VI
Valery V. Tuchin; Joseph A. Izatt; James G. Fujimoto, Editor(s)

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