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Proceedings Paper

Photothermal lifetime imaging of cell-drug interactions
Author(s): Vladimir P. Zharov; Dmitry Lapotko; Tat'yana Romanovskaya
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Paper Abstract

A new concept of photothermal (PT) lifetime imaging (PTI) is suggested. This technique is based on heating absorbing intracelular chromophores with a short laser pulse, together with time-resolved monitoring of their cooling. The process is monitored with phase-contrast imaging of a second coaxial probe pulse. PTI enables the estimation of the average size of absorbing targets by measuring their cooling times. Resolving overlapping absorption targets of varying sizes can be accomplished by adjusting the time delay between excitation and probe pulses. The pharmaceutical application of PTI is based on the assumption that drug action, through different biochemical processes, could change some properties of endogenous chromophores (absorption, sizes, etc.) as natural markers. This can lead to corresponding changes in PT signal parameters (amplitude, cooling time etc.). The ability of PTI to obtain information about a drug's impact is discussed, with emphasis on PT monitoring of alterations in the cellular absorbing ultrastructure. Preliminary experimental data are presented as PT images of blood cells in the presence of a drug obtained with a pulsed Nd:YAG laser (8 ns, 532 nm, 1-100 (mu) J). Other potential applications of PTI operating in lifetime mode include guidance of laser cellular microsurgery, visualization of local temperature effects and study of nano-scale structures.

Paper Details

Date Published: 5 June 2002
PDF: 12 pages
Proc. SPIE 4623, Functional Monitoring and Drug-Tissue Interaction, (5 June 2002); doi: 10.1117/12.469469
Show Author Affiliations
Vladimir P. Zharov, Univ. of Arkansas for Medical Sciences (United States)
Dmitry Lapotko, Luikov Heat and Mass Transfer Institute (United States)
Tat'yana Romanovskaya, Luikov Heat and Mass Transfer Institute (Belarus)

Published in SPIE Proceedings Vol. 4623:
Functional Monitoring and Drug-Tissue Interaction
Manfred D. Kessler; Gerhard J. Mueller, Editor(s)

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