Subcellular particles (mitochondria, cellular pigments etc.) are mainly responsible for light scattering in living tissues in relation to the functional state. 2-D images clarify the respective tissue status during normoxia or anoxia such as the redox state of cytochromes. We realized tissue imaging of twelve isolated perfused rat livers stained with Merocyanine-540 during normoxia, hypoxia and anoxia. Merocyanine-spectra have shown a maximum fluorescence peak at 596 +/- 2 nm. The optical response increased under desoxygenation and decreased under reoxygenation and might be correlated to electrical potential alterations. Furthermore, we record oscillations with frequencies over 7/sec (420/min) which might be correlated to intracellular processes. The additional use of dyes for tissue imaging gives us the opportunity of new insights into organ function.

Date Published: 5 June 2002
PDF: 12 pages
Proc. SPIE 4623, Functional Monitoring and Drug-Tissue Interaction, (5 June 2002); doi: 10.1117/12.469466

Published in SPIE Proceedings Vol. 4623:
Functional Monitoring and Drug-Tissue Interaction
Manfred D. Kessler; Gerhard J. Mueller, Editor(s)