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Proceedings Paper

Catchment volume and penetration depth in different suspensions of liver mitochondria monitored in scattering chambers
Author(s): Marc Dammann; Christine Mahlke; Valerie Steinbach; Michael Singer; Manfred D. Kessler
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Paper Abstract

Spectrometry is a well established method for in vitro and in vivo measurements. For interpretation of experimental results and patient monitoring it is important to regard the catchment volume and penetration depth of backscattered light. We investigated different suspensions of rat liver mitochondria as well as rat liver homogenate in a scattering chamber combined with a scanning procedure. To compare these results with a common used medium we measured Lipofundin 2% additionally. The highest light intensity caused Lipofundin 2%, followed by 100% mitochondrial concentration, liver homogenate and 50% mitochondrial concentration. Most of times the light penetrates slightly pronounced in lateral than in forward direction. Furthermore, we investigated the dependence of optical fibers with different thickness on catchment volume and penetration depth. Optical fibers were applied with a diameter of 200 micrometers and 250 micrometers respectively presenting a great increase in catchment volume and penetration depth in the larger one.

Paper Details

Date Published: 5 June 2002
PDF: 8 pages
Proc. SPIE 4623, Functional Monitoring and Drug-Tissue Interaction, (5 June 2002); doi: 10.1117/12.469440
Show Author Affiliations
Marc Dammann, Friedrich-Alexander-Univ. Erlangen-Nuernberg (Germany)
Christine Mahlke, Friedrich-Alexander-Univ. Erlangen-Nuernberg (Germany)
Valerie Steinbach, Friedrich-Alexander-Univ. Erlangen-Nuernberg (Germany)
Michael Singer, Friedrich-Alexander-Univ. Erlangen-Nuernberg (Germany)
Manfred D. Kessler, Friedrich-Alexander-Univ. Erlangen-Nuernberg (Germany)

Published in SPIE Proceedings Vol. 4623:
Functional Monitoring and Drug-Tissue Interaction
Manfred D. Kessler; Gerhard J. Mueller, Editor(s)

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