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Proceedings Paper

Chemical surface management for micro PCR in silicon chip thermocyclers
Author(s): Jana Felbel; Ivonne Bieber; Johann Michael Koehler
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Paper Abstract

Silicon, silicon dioxide, glass and other key materials of micro system technology show an inhibiting effect on PCR. This negative influence becomes seriously, if devices are miniaturized, particularly in case of flow-through devices due to their high surface to volume ratio. In contrast, alkyl-substituted surfaces do not inhibit the reaction. Although the silanization improves the compatibility, the suppression of inhibition by wall surface treatment was not stable over longer time intervals. Therefore, the stability of chemical surface modifications was studied in dependence of silanization, material, pH, temperature and buffer composition. The efficiency of surface covering by molecular substitution was characterized by wetting experiments as well as by PCR test runs. The results show that the surface treatment can be optimized by the choice of silanization agents and the concentration of surface active additives.

Paper Details

Date Published: 14 November 2002
PDF: 7 pages
Proc. SPIE 4937, Biomedical Applications of Micro- and Nanoengineering, (14 November 2002); doi: 10.1117/12.468657
Show Author Affiliations
Jana Felbel, Institute for Physical High Technology Jena (Germany)
Ivonne Bieber, Institute for Physical High Technology Jena (Germany)
Johann Michael Koehler, Institute for Physical High Technology Jena (Germany)
Technical Univ. of Illmenau (Germany)

Published in SPIE Proceedings Vol. 4937:
Biomedical Applications of Micro- and Nanoengineering
Dan V. Nicolau, Editor(s)

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