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Proceedings Paper

Preparation and characterization of phantom objects for optical imaging by time-resolved transmittance and fluorescence
Author(s): Laura Bottalico; Ines Delfino; Rosario Esposito; Maria Lepore
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Paper Abstract

Well-characterized phantom objects are necessary for investigating the performances of optical imaging systems based on time-resolved transmittance and fluorescence. For this purposes we have prepared inhomogeneous phantoms made of gelatinous objects placed in aqueous solutions of 10% Intralipid with different concentrations. The gelatinous objects have been prepared using a mixture of 10% Intralipid with agar at which absorbing ink have been added for transmittance based optical imaging system. For fluorescence measurements proper fluorescent dyes (rhodamine6G and IR125) have been added. Conventional optical characterization by spectrophotometric and spectrofluorimetric measurements have been performed. In addition, time-resolved transmittance and fluorescence measurements have been carried out. In particular, time-correlated single photon counting system has been used for time-resolved transmittance measurements. For time-resolved fluorescence measurements an optical imaging system based on a Ti:Sa laser and streak camera has been employed.

Paper Details

Date Published: 28 May 2002
PDF: 8 pages
Proc. SPIE 4622, Optical Diagnostics of Living Cells V, (28 May 2002); doi: 10.1117/12.468357
Show Author Affiliations
Laura Bottalico, INFM (Italy)
Ines Delfino, INFM (Italy)
Rosario Esposito, INFM and Univ. degli Studi di Napoli Federico II (Italy)
Maria Lepore, UNFM and Seconda Univ. degli Studi di Napoli Federico II (Italy)

Published in SPIE Proceedings Vol. 4622:
Optical Diagnostics of Living Cells V
Robert C. Leif; Daniel L. Farkas; Robert C. Leif, Editor(s)

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