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Proceedings Paper

Imaging photosensitizer distribution and pharmacology using multiphoton microscopy
Author(s): Eric A. Wachter; Craig Dees; Jay Harkins; Walter G. Fisher; Timothy Scott
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Paper Abstract

Multiphoton microscopy is a powerful tool for imaging sub- cellular distribution of luminescent compounds present in living cells. We have used this tool to study the distribution and pharmacology of photosensitizers in tissue and tissue culture. Murine hepatoma tumor cells dosed with a photosensitizer were briefly photoactivated, then imaged for periods up to several hours. Using the photosensitizer Rose Bengal with green light activation, nearly immediate photolytic release of lysosomal enzymes resulted in catastrophic cell destruction within 5 - 30 minutes. The magnitude and rapidity of this response is markedly different than that observed with other photosensitizer agents, and is consistent with in vivo studies illustrating that Rose Bengal is capable of causing extremely rapid destruction of treated tumors.

Paper Details

Date Published: 28 May 2002
PDF: 7 pages
Proc. SPIE 4622, Optical Diagnostics of Living Cells V, (28 May 2002); doi: 10.1117/12.468334
Show Author Affiliations
Eric A. Wachter, Photogen, Inc. (United States)
Craig Dees, Photogen, Inc. (United States)
Jay Harkins, Photogen, Inc. (United States)
Walter G. Fisher, Photogen, Inc. (United States)
Timothy Scott, Photogen, Inc. (United States)

Published in SPIE Proceedings Vol. 4622:
Optical Diagnostics of Living Cells V
Robert C. Leif; Daniel L. Farkas; Robert C. Leif, Editor(s)

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