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Proceedings Paper

Scanning aperture polarized light microscope: observation of small calcite crystals using oblique illumination
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Paper Abstract

The article presents a new design of a polarization microscope with a scanning liquid crystal aperture. The scanning device is based on the earlier reported Pol-Scope technique and includes a liquid crystal universal compensator. It is mounted in the front focal plane of the high numerical aperture condenser lens on the microsccope. By occluding different portions of the aperture, an oblique beam of variable tilt angle and azimuth is created for illuminating the specimen. Birefringence measurements are recorded for different mask configurations and results are evaluated to determine the retardance magnitude, azimuth and direction of optic axis of the specimen. We report measurements using small calcite crystals that confirm our theoretical predictions.

Paper Details

Date Published: 15 May 2002
PDF: 6 pages
Proc. SPIE 4621, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX, (15 May 2002); doi: 10.1117/12.467840
Show Author Affiliations
Michael I. Shribak, Marine Biological Lab. (United States)
Rudolf Oldenbourg, Marine Biological Lab. (United States)

Published in SPIE Proceedings Vol. 4621:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing IX
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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