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Proceedings Paper

Two-photon fluorescence imaging of embryo with much less damage than confocal imaging
Author(s): Bian Liu; Hui Xu; Lei Jin; Hui Ma; Die Yan Chen
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Paper Abstract

Two-photon Laser Scanning Microscopy (TPLSM) is a novel technique based on the two-photon excitation of fluorophore. In this paper, TPLSM and traditional confocal microscopy are introduced. And the influence of femtosecond near-infrared (NIR) illumination on mouse embryos is investigated for the first time. The result shows that NIR laser has much less damage to embryos than blue laser and proves that TPLSM is superior to conventional confocal microscopy in keeping sample alive. TPLSM enables us to make a continuous observation for a longer time on embryogenesis.

Paper Details

Date Published: 20 September 2002
PDF: 4 pages
Proc. SPIE 4919, Advanced Materials and Devices for Sensing and Imaging, (20 September 2002); doi: 10.1117/12.465727
Show Author Affiliations
Bian Liu, Tsinghua Univ. (China)
Hui Xu, Tsinghua Univ. (China)
Lei Jin, Tsinghua Univ. (China)
Hui Ma, Tsinghua Univ. (China)
Die Yan Chen, Tsinghua Univ. (China)

Published in SPIE Proceedings Vol. 4919:
Advanced Materials and Devices for Sensing and Imaging
Jianquan Yao; Yukihiro Ishii, Editor(s)

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