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Proceedings Paper

Imaging and measurement of cell structure and organization with submicron accuracy using light scattering spectroscopy
Author(s): Vadim Backman; Rajan S. Gurjar; Lev T. Perelman; Venkatesh Gopal; Maxim Kalashnikov; Kamran Badizadegan; Adam Wax; Irene Georgakoudi; Markus G. Mueller; Charles W. Boone; Irving Itzkan; Ramachandra R. Dasari; Michael S. Feld
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Paper Abstract

Light scattering spectroscopy (LSS) is a promising optical technique developed for quantitative characterization of tissue morphology as well as in vivo detection and diagnosis of disease such as early cancer. LSS employs a wavelength dependent component of light scattered by epithelial cells and other tissues to obtain information about subcellular structure. We present two novel modalities of LSS, LSS imaging and scattering angle sensitive LSS (a/LSS). LSS imaging provides quantitative information about the epithelial cell nuclei, such as nuclear size, degree of pleomorphism, hyperchromasia, and amount of chromatin. It allows mapping these histological properties over wide areas of epithelial lining. We show that LSS imaging can be used to detect precancerous lesions in optically accessible organs. Using a/LSS, which enables characterization of tissue components with sizes smaller than the wavelength of light, we show that the number of subcellular components with the sizes between 30 nm and few microns scales with the size according to an inverse power-law. We show that the size distribution exponent is an important parameter characterizing tissue organization, for example the balance between stochasticity and order, and has a potential to be applicable for early cancer diagnosis and characterization.

Paper Details

Date Published: 1 May 2002
PDF: 10 pages
Proc. SPIE 4613, Optical Biopsy IV, (1 May 2002); doi: 10.1117/12.465234
Show Author Affiliations
Vadim Backman, Northwestern Univ. (United States)
Rajan S. Gurjar, Massachusetts Institute of Technology (United States)
Lev T. Perelman, Harvard Medical School (United States)
Venkatesh Gopal, Massachusetts Institute of Technology (India)
Maxim Kalashnikov, Massachusetts Institute of Technology (United States)
Kamran Badizadegan, Children's Hospital/Harvard Medical School (United States)
Adam Wax, Massachusetts Institute of Technology (United States)
Irene Georgakoudi, Massachusetts Institute of Technology (United States)
Markus G. Mueller, Massachusetts Institute of Technology (United States)
Charles W. Boone, Massachusetts Institute of Technology (United States)
Irving Itzkan, Northwestern Univ. (United States)
Ramachandra R. Dasari, Massachusetts Institute of Technology (United States)
Michael S. Feld, Massachusetts Institute of Technology (United States)

Published in SPIE Proceedings Vol. 4613:
Optical Biopsy IV
Robert R. Alfano, Editor(s)

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