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Proceedings Paper

Molecular information from fluorescence spectroscopic investigations of breast tissues and tissue phantoms
Author(s): Maya S. Nair; Sharad Gupta; Uvija Sinha; Asima Pradhan
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Paper Abstract

Polarized fluorescence spectroscopy in the visible range can be used to discriminate different types of human breast tissue, namely, malignant, benign and normal. The bandwidth of the 580nm band of the polarized spectra shows excellent discrimination among the different tissue types. This difference in the bandwidth of the 580nm band provides bio-molecular information of the breast tissues. The spatially dependent fluorescence spectra are recorded from different radial distances of the tissue sample. These spectral profiles show differences in broadening as one goes away from the excitation point. This broadening also provides information on the presence of porphyrin in the breast tissues. Various concentrations of two fluorophores with varying concentrations of scatterers have been studied based on the assumption that porphyrin plays a major role in progress of tumor. Correlations have been made with polarized fluorescence spectra as well as spatial profiles of fluorescence spectra of the different tissues with the measurements of tissue phantoms. Such correlations hint at porphyrin accumulation and scattering effects playing major roles in spectral differences among normal, benign and malignant tumor breast tissues.

Paper Details

Date Published: 1 May 2002
PDF: 6 pages
Proc. SPIE 4613, Optical Biopsy IV, (1 May 2002); doi: 10.1117/12.465231
Show Author Affiliations
Maya S. Nair, Indian Institute of Technology (India)
Sharad Gupta, Indian Institute of Technology (India)
Uvija Sinha, Indian Institute of Technology (India)
Asima Pradhan, Indian Institute of Technology (India)

Published in SPIE Proceedings Vol. 4613:
Optical Biopsy IV
Robert R. Alfano, Editor(s)

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