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Proceedings Paper

Characterization of biofilm components using a fiber optic spectrometer
Author(s): Markus Leitz; R. Sekar; T. Griebe; S. Krautwald; Hilmar Franke
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Paper Abstract

A simple technique for the optical characterization and detection of early stages of biofilm is presented. A bacterial biofilm was developed in a microcell, consisting of of two glass slides and the biofilm between the slides. This technique allowed the measurement of biofilm thickness and refractive index of the medium as a function of position and time. Using a sensititive broad bandgap foil as surface, the early stage of biofilm formation was investigated in a simple reflectivity measurement using a fiber optic spectrometer. The optical results were compared with the measurements of confocal laser scanning microscope (CLSM). The fiber optic spectrometer technique was suitable for the detection of low bacterial densities (104 cm-2).

Paper Details

Date Published: 26 March 2002
PDF: 9 pages
Proc. SPIE 4616, Optical Fibers and Sensors for Medical Applications II, (26 March 2002); doi: 10.1117/12.463820
Show Author Affiliations
Markus Leitz, Gerhard-Mercator-Univ.-Gesamthochschule Duisburg (Germany)
R. Sekar, Gerhard-Mercator-Univ.-Gesamthochschule Duisburg (Germany)
T. Griebe, Gerhard-Mercator-Univ.-Gesamthochschule Duisburg (Germany)
S. Krautwald, Gerhard-Mercator-Univ.-Gesamthochschule Duisburg (Germany)
Hilmar Franke, Gerhard-Mercator-Univ.-Gesamthochschule Duisburg (Germany)

Published in SPIE Proceedings Vol. 4616:
Optical Fibers and Sensors for Medical Applications II
Israel Gannot, Editor(s)

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