Share Email Print

Proceedings Paper

Potential of Raman spectroscopy for the molecular characterization of human tumors
Author(s): Eoghan O. Faolain; Jennifer Conroy; Mary Hunter; Peter Kelehan; Helen Lambkin; Carmel Mothersill; Hugh James Byrne; Fiona M. Lyng
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

In this study Raman spectroscopy was employed distinguish between normal and abnormal human tissue. Raman spectra were obtained using a confocal Raman microspectrometer and a laser excitation of 514.532nm for a number of healthy tissues (Tonsil and Cervix) as well as for their diseased counterparts. Each tissue type was classified and its various spectral components were associated with vibrations, rotations etc. of the chemical bonds in the tissues' constituent components. Raman bands have been attributed to biological compounds such as proteins, lipids and DNA as well as to chemical bonds within these compounds such as, C=O stretch in Amide I (1645-1680 cm-1), NH bending in Amide III (1230-1310 cm-1), phenyl ring in Phenylalanine (1004 cm-1). Changes in the relative intensities, position, and width of these bands relate to the composition of the sample. Differences between healthy and diseased tissue with progression to malignancy have been identified and include; an increase in intensity of the phenylalanine ring breathing band (1004 cm-1), the C-C stretch of proteins (1082 cm-1), Amide I band (1655 cm-1) and the C-N stretching modes of proteins (1082cm-1). Decreases in relative intensities were also observed, and include a decrease in intensity of the CH2 deformation (1295 cm-1), CH2 bending of proteins and lipids (1445 cm-1), and the C=O stretching of proteins (1695 cm-1). The molecular origin of the observed spectral changes is discussed.

Paper Details

Date Published: 27 August 2003
PDF: 8 pages
Proc. SPIE 4876, Opto-Ireland 2002: Optics and Photonics Technologies and Applications, (27 August 2003); doi: 10.1117/12.463630
Show Author Affiliations
Eoghan O. Faolain, Dublin Institute of Technology (Ireland)
Jennifer Conroy, Dublin Institute of Technology (Ireland)
Mary Hunter, National Maternity Hospital (Ireland)
Peter Kelehan, National Maternity Hospital (Ireland)
Helen Lambkin, Dublin Institute of Technology (Ireland)
Carmel Mothersill, Dublin Institute of Technology (Ireland)
Hugh James Byrne, Dublin Institute of Technology (Ireland)
Fiona M. Lyng, Dublin Institute of Technology (Ireland)

Published in SPIE Proceedings Vol. 4876:
Opto-Ireland 2002: Optics and Photonics Technologies and Applications
Vincent Toal; Norman Douglas McMillan; Gerard M. O'Connor; Eon O'Mongain; Austin F. Duke; John F. Donegan; James A. McLaughlin; Brian D. MacCraith; Werner J. Blau, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?