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Proceedings Paper

A novel flip-chipped integrated single photon detection platform for proteomics
Author(s): J. Carlton Jackson; Jonathan West; Aoife M. Moloney; Dan O'Connell; Alan P. Morrison; Alan Mathewson
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Paper Abstract

Novel integrated sensors will be required for future detection platforms for the measurement of fluorescence and luminescence. The current trend towards integration of optical detectors and the broad advances in optical emitting dyes and proteins will be combined in robust, low-cost, point-of-use, diagnostic equipment. To this end we are experimenting with an integrated optical hybrid sensing device which will combine a flip-chipped, array of solid-state single photon counting detectors with surface mount passive quench circuits on a conventional glass substrate. This flip-chipped arrangement both 1) increases the speed of response of the detector and 2) increases the robustness and ease of integration and reduces single photon detector handling requirements. The potential of integrated solid-state photon detectors will be demonstrated for the real-time quantitative detection of luciferase, a light emitting protein expression reporter molecule. A 15μm solid-state Geiger-mode avalanche photodiode (APD) operating in single photon counting mode will be compared with a standard photomultiplier tube (PMT) for luciferase luminescence detection. Detection levels of 2×106 and 1×107 enzyme molecules will be demonstrated for PMT and Geiger-mode APD respectively. The size of the Geiger-mode APD active area will be shown to be the limiting factor in luciferase signal detection for non-integrated applications. A simple geometric model will show that detection limits of 1×104 are achievable in integrated sensing platforms using room temperature operated single photon counting detectors.

Paper Details

Date Published: 27 August 2003
PDF: 11 pages
Proc. SPIE 4876, Opto-Ireland 2002: Optics and Photonics Technologies and Applications, (27 August 2003); doi: 10.1117/12.463623
Show Author Affiliations
J. Carlton Jackson, National Microelectronics Research Ctr. (Ireland)
Jonathan West, National Microelectronics Research Ctr. (Ireland)
Aoife M. Moloney, Univ. College Cork (Ireland)
Dan O'Connell, National Microelectronics Research Ctr. (Ireland)
Alan P. Morrison, Univ. College Cork (Ireland)
Alan Mathewson, National Microelectronics Research Ctr. (Ireland)

Published in SPIE Proceedings Vol. 4876:
Opto-Ireland 2002: Optics and Photonics Technologies and Applications
Vincent Toal; Norman Douglas McMillan; Gerard M. O'Connor; Eon O'Mongain; Austin F. Duke; John F. Donegan; James A. McLaughlin; Brian D. MacCraith; Werner J. Blau, Editor(s)

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