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Proceedings Paper

Effect of low-power HeNe laser irradiation in vitro on intracellular calcium concentration and cellular activity in mouse macrophages
Author(s): Chaoyang Xu; Shici Tan; Da Xing
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Paper Abstract

Low level He-Ne laser irradiation (e.g., intravascular laser irradiation on blood, ILIB) has been widely used to treat some illness clinically. One of the action processing is by improving immunocompetence of the body, but the mechanism is still not clear. In this paper, we used mouse macrophages as study object for real-time observation of the concentration change of intracellular free calcium (abb. as [Ca2+]i) of macrophages and the distribution of the Ca2+ using LSCM (laser scanning confocal microscope) after irradiation with different dose low level He-Ne laser (the actual irradiation power (abb. as P) is 0.16 mw and the facular diameter (abb. as D) is 0.5 cm. We found interesting phenomena through the image of [Ca2+]i of macrophages. The distribution of Ca2+ in macrophages has no obvious change when the time of irradiation (abb. as Ti) is 10 min. The [Ca2+]i shows obvious increase and a concentrate phenomena in Ca2+ distribution appears when Ti reach 20 min. The change of the distribution of Ca2+ become more distinct: [Ca2+]i increase more visibly and the distribution of Ca2+ shows a spacial grads when Ti reach 30 min.

Paper Details

Date Published: 12 April 2002
PDF: 5 pages
Proc. SPIE 4536, International Workshop on Photonics and Imaging in Biology and Medicine, (12 April 2002); doi: 10.1117/12.462543
Show Author Affiliations
Chaoyang Xu, South China Normal Univ. (China)
Shici Tan, South China Normal Univ. (China)
Da Xing, South China Normal Univ. (China)

Published in SPIE Proceedings Vol. 4536:
International Workshop on Photonics and Imaging in Biology and Medicine
Qingming Luo; Britton Chance; Valery V. Tuchin, Editor(s)

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